Green Synthesis of Selenium Nanoparticles Utilizing <i>Drimia indica</i>: Insights Into Anticancer and Antimicrobial Activities

Selenium nanoparticles (SeNPs) have garnered significant interest as anticancer and antimicrobial agents. The aqueous extract of medicinal plant Drimia indica leaves (DI-LAE) was used to synthesize SeNPs (DI-SeNPs) that were extensively characterized by UV-visible absorbance, TEM, EDX, XRD, zeta potential measurements, and FTIR. DI-SeNPs exhibited dose-dependent toxicity against the human lung adenocarcinoma cell line (A549; IC₅₀ of 43.21 μg/mL). DI-SeNPs increased reactive oxygen species (ROS) generation in A549 cells. DI-SeNPs caused cell cycle arrest in the G2/M phase and increased DNA damage in A549 cells, ultimately driving these cells toward apoptosis. DI-SeNPs significantly increased p53 levels, decreasing Akt levels and elevating cleaved caspase 3 levels in A549 cells. Additionally, DI-SeNPs exhibited antimicrobial activity against various bacteria and fungi. These findings suggest that DI-SeNPs possess significant anticancer and antimicrobial properties, mediated through mechanisms involving ROS generation, cell cycle arrest, and apoptosis induction.