Screening of marcescin producing isolates from Serratia marcescens

The current study intends to investigate the production of bacteriocin from S. marcescens, a Gram-negative bacterium, is a human pathogen and the reason why hospital medical equipment becomes contaminated. Various samples from patients about 220 samples which include blood,urine,abscess and sputum, twenty -three isolates of S.marcescens was performed as per standard laboratory procedure and confirmed by using VITEK-2 systems. The isolates of S.marcescens which produced marcescin detected by four methods : spot –on-the –lawn method , Cup-assay method ,Well-diffusion method and Agar cross streaking assay , the production of bacteriocin was produced by adding mitomycin-c to the bacterial broth to increase the amount of bacteriocin.