Spns1-dependent endocardial lysosomal function drives valve morphogenesis through Notch1-signaling

Autophagy-lysosomal degradation is a conserved homeostatic process considered to be crucial for cardiac morphogenesis. However, both its cell specificity and functional role during heart development remain unclear. Here, we introduced zebrafish models to visualize autophagic vesicles <i>in vivo</i> and track their temporal and cellular localization in the larval heart. We observed a significant accumulation of autolysosomal and lysosomal vesicles in the atrioventricular and bulboventricular regions and their respective valves. We addressed the role of lysosomal degradation based on the Spinster homolog 1 (<i>spns1</i>) mutant (<i>not really started</i>, <i>nrs</i>). <i>n</i> <i>rs</i> larvae displayed morphological and functional cardiac defects, including abnormal endocardial organization, impaired valve formation and retrograde blood flow. Single-nuclear transcriptome analyses revealed endocardial-specific differences in lysosome-related genes and alterations of <i>notch1-</i>signalling. Endocardial-specific overexpression of <i>spns1</i> and <i>notch1</i> rescued features of valve formation and function. Altogether, our results reveal a cell-autonomous role of lysosomal processing during cardiac valve formation affecting <i>notch1-</i>signalling.