Analysis of the regulation mechanism for salt-tolerant anammox process: process performance and metabolic insights

In this study, the start-up and microbial domestication of a salt-tolerant functional anammox system was investigated by gradually increasing the salinity level in a stabilized anammox system in the laboratory. After 44 days of stable operation, the salt-tolerant system was successfully activated, at which time the salinity of the influent water was 3 g/L, and the maximum removal efficiency of ammonia nitrogen and nitrite nitrogen in the system reached 94.18% and 96.66%, respectively, and then the ammonia nitrogen and nitrite nitrogen removal efficiency were stabilized at 88.17% and 96.48% after the enrichment domestication for 89 days. The system was operated in the salinity of 10 g/L, with the concentration of each nitrogen compound measured at the same time. The ammonia nitrogen removal efficiency decreased to 59.93% at a salinity of 10 g/L, which had a significant impact on the system. High-throughput sequencing revealed that the system was enriched with a large number of <i>Chloroflexi</i>, the relative abundance of which increased from 19.46% to 52.33%, and the genus of AnAOB was transformed from <i>Candidatus Brocadia</i> to <i>Candidatus Kuenenia, Candidatus Kuenenia</i>, with a percentage of 4.78%. The system successfully achieved the simultaneous removal of ammonia nitrogen and nitrite nitrogen under salinity stress, which to a certain extent indicated that AnAOB could achieve the initiation and enrichment domestication under salinity conditions, and could provide a basis for the efficient and low-consumption treatment of high salinity nitrogen-containing wastewater.<b>Highlights</b>Anammox reaction can be successfully initiated under low salinity conditionsSalinity of 10 g/L has a severe shock effect on the anammox systemAfter salinity enrichment and domestication, the abundance of norank_f__norank__o___SBR1031 and Candidatus Kuenenia in the anammox reaction reached 26.7% and 4.78%, respectively.