Collateral Sensitivity to β-Lactam Antibiotics in Evolved Apramycin-Resistant MRSA
Collateral sensitivity is an evolutionary trade-off for bacteria where acquiring resistance to one antibiotic results in an increased sensitivity to another antibiotic. This study was designed to evaluate the collateral sensitivity of methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) to β-lactam antibiotics induced by the evolution of resistance to apramycin. Collateral sensitivity to ampicillin, cephazolin, ceftriaxone, cefotaxime, cefepime and cefquinome occurred after MRSA were exposed to apramycin and induced to acquire resistance. This sensitivity was associated with reduced β-lactamase activity and decreased expression of the <i>mecA</i> gene. We also found a decrease in the proton motive force and decreased efflux activity. These results provide new insights into collateral sensitivity-based strategies for the treatment of MRSA.
Authors
- Juan Liu (ORCID: https://orcid.org/0000-0002-6980-5945)
- Changmin Li (ORCID: https://orcid.org/0000-0002-1078-8570)
- Shiqian Wu (ORCID: https://orcid.org/0000-0002-6383-7663)
- Yang Yu (ORCID: https://orcid.org/0000-0001-9052-1245)
- Yan Zhang (ORCID: https://orcid.org/0000-0002-2947-1629)
- Mei Zheng
- Fu-Hao Li
- Jingjing Wu (ORCID: https://orcid.org/0009-0006-0206-7876)
- Yashuang Wu
Institutions
- South China Agricultural University (CN)
- Guangzhou University of Chinese Medicine (CN)
Publication Details
- Journal
- International Journal of Molecular Sciences
- Published
- 2024-11-15
- DOI
- https://doi.org/10.3390/ijms252212292
- Primary Topic
- Antibiotics Pharmacokinetics and Efficacy
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Guangdong Province
- Foundation for Innovative Research Groups of the National Natural Science Foundation of China
- Guangdong Provincial Pearl River Talents Program
- National Key Research and Development Program of China
- Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center)