Molecular profiles, sources and lineage restrictions of stem cells in an annelid regeneration model
Regeneration of missing body parts can be observed in diverse animal phyla, but it remains unclear to which extent these capacities rely on shared or divergent principles. Research into this question requires detailed knowledge about the involved molecular and cellular principles in suitable reference models. By combining single-cell RNA sequencing and mosaic transgenesis in the marine annelid Platynereis dumerilii, we map cellular profiles and lineage restrictions during posterior regeneration. Our data reveal cell-type specific injury responses, re-expression of positional identity factors, and the re-emergence of stem cell signatures in multiple cell populations. Epidermis and mesodermal coelomic tissue produce distinct putative posterior stem cells (PSCs) in the emerging blastema. A novel mosaic transgenesis strategy reveals both developmental compartments and lineage restrictions during regenerative growth. Our work supports the notion that posterior regeneration involves dedifferentiation, and reveals molecular and mechanistic parallels between annelid and vertebrate regeneration.
Authors
- Leonie Adelmann (ORCID: https://orcid.org/0009-0003-7223-819X)
- Nadja Milivojev (ORCID: https://orcid.org/0009-0003-0340-4713)
- Martin Fahrenberger (ORCID: https://orcid.org/0000-0003-1107-0197)
- Christine Ruta (ORCID: https://orcid.org/0000-0001-6412-6751)
- B. Duygu Özpolat (ORCID: https://orcid.org/0000-0002-1900-965X)
- Alexander W. Stockinger (ORCID: https://orcid.org/0000-0003-2058-2813)
- Florian Raible (ORCID: https://orcid.org/0000-0002-4515-6485)
- Guillaume Balavoine (ORCID: https://orcid.org/0000-0003-0880-1331)
Institutions
- Universidade Federal do Rio de Janeiro (BR)
- Centre National de la Recherche Scientifique (FR)
- University of Vienna (AT)
- Washington University in St. Louis (US)
- Université Paris Cité (FR)
- Université Paris-Saclay (FR)
- Max Perutz Labs (AT)
- Institut des Neurosciences Paris-Saclay (FR)
- Institut Jacques Monod (FR)
- Vienna Biocenter (AT)
- Austrian Cluster for Tissue Regeneration (AT)
- Medical University of Vienna (AT)
Publication Details
- Journal
- Nature Communications
- Published
- 2024-11-18
- DOI
- https://doi.org/10.1038/s41467-024-54041-3
- Citations
- 8
- Primary Topic
- Marine Ecology and Invasive Species
- Type
- article
- Field-Weighted Citation Impact
- 2.26
Funders
- Agence Nationale de la Recherche
- Österreichischen Akademie der Wissenschaften
- Austrian Science Fund
- Universität Wien
- Fondation ARC pour la Recherche sur le Cancer
- Studienstiftung des Deutschen Volkes