Beginner’s guide to investigating protein: DNA interactions using electrophoretic mobility shift assays (EMSAs)

This article describes how DNA molecules typically migrate through a gel/matrix. It describes the materials required to perform electrophoretic mobility shift assays (EMSAs), the methods used to set up a reaction and components that require optimization. It also shines light on the applications of EMSA, including appropriate controls that can make it more interpretive and alternatives to EMSA if the user requires more specialization.