Resistance gene enrichment sequencing for NLR genes for <i>Phytophthora sojae</i> in selected soybean plant introductions and differentials with putative novel and known <i>Rps</i> genes

Abstract Numerous sources of putative novel resistance genes toward Phytophthora sojae ( Rps genes) have been identified and loci mapped in soybean ( Glycine max L. Merr.) but cloning has remained elusive. We utilized resistance gene enrichment sequencing (RenSeq) to identify the putative resistance genes in 20 plant introductions (PIs) and differentials of the cultivar Williams with rps , Rps1c , Rps3a , and Rps8 . The DNA from these genotypes was enriched and sequenced using more than 25,000 80 nt baits designed to nucleotide‐binding leucine‐rich repeat (NLR) encoding sequences. Overall, there were greater numbers of variants in the NLR‐encoding genes in Rps loci on chromosomes (Chrs) 3, 7, 13, and 18 for the 20 PIs as compared to the Williams differentials for rps , Rps1c , Rps1k , Rps3a , and Rps8 . Genes encoding Rps1c , Rps3a , and Rps8 were proposed based on sequence differences among the differentials. Among the 20 PIs, there may be additional alleles on Chrs 3, 13, and 18, and PI399079 may have two new alleles at Chrs 3 and 7 loci. A unique NLR on Chr 8 was identified in PI200553. New alleles were also identified on Chrs 3 and 18 when the PI and resistant bulks were compared to susceptible recombinant inbred lines. This study demonstrates the utility of RenSeq as an efficient method to identify and predict specific novel NLR genes in landrace soybean germplasm, which confer resistance to P. sojae and obtain gene‐specific markers to facilitate their introgression into modern cultivars.