Application of Pan-Viral Metagenomic Sequencing on United States Air Force Academy Wastewater to Uncover Potential Causes of Acute Gastroenteritis
Although gastroenteritis outbreaks at USAFA are commonly attributed to norovirus because of clinical presentation and the acute nature of the illness-often diagnosed and treated without confirmatory stool testing-this virus was not found in high prevalence in these wastewater samples. Among adenoviruses, F serotype 41 predominated, suggesting a role in gastrointestinal infections among the cadet population. Phylogenetic investigation of adenovirus and norovirus exposed common variants with seasonal distributions. These findings may prompt correlational studies to assess the clinical predictive capability of VirCapSeq-VERT and to determine the utility of wastewater monitoring as an outbreak early warning system.
Authors
- John P. Collins (ORCID: https://orcid.org/0000-0002-5957-6923)
- Bryant J. Webber (ORCID: https://orcid.org/0000-0002-2099-2671)
- Armand L Balboni (ORCID: https://orcid.org/0000-0002-2381-9911)
- Alper Gokden (ORCID: https://orcid.org/0000-0002-9659-7352)
- Michael A. Mechikoff
- J. Kenneth Wickiser (ORCID: https://orcid.org/0000-0002-5034-2100)
- Andrew J. Wallace (ORCID: https://orcid.org/0000-0002-9603-8892)
- Celeste Ingersoll (ORCID: https://orcid.org/0000-0002-7586-2866)
- J. Jordan Steel (ORCID: https://orcid.org/0000-0002-1256-2639)
- Xiang‐Jun Lu (ORCID: https://orcid.org/0000-0002-8901-7444)
- Philip M Golder
- Riley E McGarry
- Anastasia Cuff
Institutions
- United States Army Medical Command (US)
- United States Air Force Academy (US)
- Columbia University (US)
Publication Details
- Journal
- Military Medicine
- Published
- 2024-11-15
- DOI
- https://doi.org/10.1093/milmed/usae518
- Citations
- 4
- Primary Topic
- Viral gastroenteritis research and epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 1.56
Funders
- Skoll Foundation
- Defense Health Agency
- Air Force Office of Scientific Research