DETERMINATION OF E PROTEIN MUTATION IN SARS-COV-2 VIRUS ISOLATES

The emergence of new variants of the SARS-CoV-2 coronavirus is caused by mutations in the main structural proteins of severe acute respiratory syndrome coronavirus 2. Vaccination and other therapeutic approaches can help stop the epidemic. Scientists are currently developing drugs and vaccines that specifically target the structural proteins of the SARS-CoV-2 coronavirus. As a result, taking into account mutations in proteins and determining their impact on function will help in high-quality production and development of preventive and therapeutic agents. As a result of sequencing, it was possible to obtain the complete nucleotide sequence of the E gene of the SARS-CoV-2 coronavirus. The presence and location of mutations in the envelope protein (E) of SARS-CoV-2 virus isolates were investigated by aligning the sequences with the reference sequence of the SARS-CoV-2 virus. The results showed that the most relative mutations in the amino acid sequence of the E protein of the SARS-CoV-2 coronavirus occurred in regions 9 and 11. Two mutations, T9I and T11A, were found compared with the Severe acute respiratory syndrome coronavirus 2 (NC 045512.2) strain. The identified structural mutations of the E protein can be used in the strategy for developing drugs and vaccines.