MosAIC: An annotated collection of mosquito-associated bacteria with high-quality genome assemblies

Mosquitoes transmit medically important human pathogens, including viruses like dengue virus and parasites such as Plasmodium spp., the causative agent of malaria. Mosquito microbiomes are critically important for the ability of mosquitoes to transmit disease-causing agents. However, while large collections of bacterial isolates and genomic data exist for vertebrate microbiomes, the vast majority of work in mosquitoes to date is based on 16S rRNA gene amplicon data that provides limited taxonomic resolution and no functional information. To address this gap and facilitate future studies using experimental microbiome manipulations, we generated a bacterial Mosquito-Associated Isolate Collection (MosAIC) consisting of 392 bacterial isolates with extensive metadata and high-quality draft genome assemblies that are publicly available, both isolates and sequence data, for use by the scientific community. MosAIC encompasses 142 species spanning 29 bacterial families, with members of the Enterobacteriaceae comprising 40% of the collection. Phylogenomic analysis of 3 genera, Enterobacter, Serratia, and Elizabethkingia, reveal lineages of mosquito-associated bacteria isolated from different mosquito species in multiple laboratories. Investigation into species' pangenomes further reveals clusters of genes specific to these lineages, which are of interest for future work to test for functions connected to mosquito host association. Altogether, we describe the generation of a physical collection of mosquito-associated bacterial isolates, their genomic data, and analyses of selected groups in context of genome data from closely related isolates, providing a unique, highly valuable resource for research on bacterial colonisation and adaptation within mosquito hosts. Future efforts will expand the collection to include broader geographic and host species representation, especially from individuals collected from field populations, as well as other mosquito-associated microbes, including fungi, archaea, and protozoa.

Authors

Institutions

Publication Details

Journal
PLoS Biology
Published
2024-11-15
DOI
https://doi.org/10.1371/journal.pbio.3002897
Citations
10
Primary Topic
Insect symbiosis and bacterial influences
Type
article
Field-Weighted Citation Impact
5.40

Funders

Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
OCT
article

MosAIC: An annotated collection of mosquito-associated bacteria with high-quality genome assemblies

Marcelo Jacobs‐Lorena, Kerri L. Coon, Doug E. Brackney, Sarah M. Short et al.
10 citations
PLoS Biology
Insect symbiosis and bacterial influences
5.40
article

MosAIC: An annotated collection of mosquito-associated bacteria with high-quality genome assemblies

Marcelo Jacobs‐Lorena, Kerri L. Coon, Doug E. Brackney, Sarah M. Short, Aidan Foo, Laura E. Brettell, Grant L. Hughes, Eva Heinz, David J. Lampe, Michael Hutchinson, Ananya Ferdous Hoque, Claire Valiente Moro, Michael Povelones, Miguel Medina Muñoz, Timothy D. Paustian, Blaire Steven, Holly L. Nichols, Vishaal Dhokiya, Eric P. Caragata, Michelle R. Rondon, Edwige Martin, Jessica A. Lysne, Jiannong Xu
article en
10 citations

Abstract

Mosquitoes transmit medically important human pathogens, including viruses like dengue virus and parasites such as Plasmodium spp., the causative agent of malaria. Mosquito microbiomes are critically important for the ability of mosquitoes to transmit disease-causing agents. However, while large collections of bacterial isolates and genomic data exist for vertebrate microbiomes, the vast majority of work in mosquitoes to date is based on 16S rRNA gene amplicon data that provides limited taxonomic resolution and no functional information. To address this gap and facilitate future studies using experimental microbiome manipulations, we generated a bacterial Mosquito-Associated Isolate Collection (MosAIC) consisting of 392 bacterial isolates with extensive metadata and high-quality draft genome assemblies that are publicly available, both isolates and sequence data, for use by the scientific community. MosAIC encompasses 142 species spanning 29 bacterial families, with members of the Enterobacteriaceae comprising 40% of the collection. Phylogenomic analysis of 3 genera, Enterobacter, Serratia, and Elizabethkingia, reveal lineages of mosquito-associated bacteria isolated from different mosquito species in multiple laboratories. Investigation into species' pangenomes further reveals clusters of genes specific to these lineages, which are of interest for future work to test for functions connected to mosquito host association. Altogether, we describe the generation of a physical collection of mosquito-associated bacterial isolates, their genomic data, and analyses of selected groups in context of genome data from closely related isolates, providing a unique, highly valuable resource for research on bacterial colonisation and adaptation within mosquito hosts. Future efforts will expand the collection to include broader geographic and host species representation, especially from individuals collected from field populations, as well as other mosquito-associated microbes, including fungi, archaea, and protozoa.

PLoS BiologyVol. 22(11)
New Mexico State University (US), Université Claude Bernard Lyon 1 (FR), Centre National de la Recherche Scientifique (FR), Pennsylvania Department of Environmental Protection (US), University of Wisconsin–Madison (US), Johns Hopkins University (US), Duquesne University (US), University of Strathclyde (GB), Liverpool School of Tropical Medicine (GB), Connecticut Agricultural Experiment Station (US), University of Florida (US), Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR), Pennsylvania Department of Agriculture (US), VetAgro Sup (FR), Florida Medical Entomology Laboratory (US), The Ohio State University (US), University of Salford (GB), University of Pennsylvania (US)
National Science Foundation, U.S. Department of Agriculture, Bill and Melinda Gates Foundation, Wellcome, Wellcome Trust, UK Research and Innovation, Liverpool School of Tropical Medicine, National Institute for Health and Care Research, Royal Society, National Institutes of Health, Directorate for Biological Sciences, Medical Research Council, Engineering and Physical Sciences Research Council, Biotechnology and Biological Sciences Research Council
Life in Land
Openalex Percentile: Top 4%
Insect symbiosis and bacterial influences
5.40
DOIOpen Access PDFOpenAlex
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.