Technical challenges of intracellular flow cytometry-based assays as a functional complement to diagnosis of signaling defects of inborn errors of immunity: PI3K pathway as a case of study

We highlight the importance of B cell stimulation through B cell receptor signaling, which is highly recommended, especially for samples that would be processed more than 24 hours after blood extraction. Also, having a defined experimental procedure is important, including the cytometer setup, which allows cytometer reproducibility for a period of time, enabling the comparison of a sample at different times.

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Publication Details

Journal
Frontiers in Immunology
Published
2024-11-15
DOI
https://doi.org/10.3389/fimmu.2024.1476218
Citations
3
Primary Topic
Immunodeficiency and Autoimmune Disorders
Type
article
Field-Weighted Citation Impact
0.72

Funders

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article

Technical challenges of intracellular flow cytometry-based assays as a functional complement to diagnosis of signaling defects of inborn errors of immunity: PI3K pathway as a case of study

Keren Reche Yebra, Arnaldo Bernal, Yolanda Soto Serrano, Javier Gonzalo Ocejo-Vinyals et al.
3 citations
Frontiers in Immunology
Immunodeficiency and Autoimmune Disorders
0.72
article

Technical challenges of intracellular flow cytometry-based assays as a functional complement to diagnosis of signaling defects of inborn errors of immunity: PI3K pathway as a case of study

Keren Reche Yebra, Arnaldo Bernal, Yolanda Soto Serrano, Javier Gonzalo Ocejo-Vinyals, Rebeca Rodríguez Pena, Carmen L. Avendaño-Monje, Lucía del Pino Molina, Eduardo López Granados
article en
3 citations

Abstract

We highlight the importance of B cell stimulation through B cell receptor signaling, which is highly recommended, especially for samples that would be processed more than 24 hours after blood extraction. Also, having a defined experimental procedure is important, including the cytometer setup, which allows cytometer reproducibility for a period of time, enabling the comparison of a sample at different times.

Frontiers in ImmunologyVol. 15
Hospital Universitario La Paz (ES), Hospital La Paz Institute for Health Research (ES), Centre for Biomedical Network Research on Rare Diseases (ES), Instituto de Investigación Marqués de Valdecilla (ES), Hospital Universitario Central de Asturias (ES)
Fundación Mutua Madrileña, Fundació la Marató de TV3, Instituto de Salud Carlos III
Good health and well-being
Openalex Percentile: Top 28%
Immunodeficiency and Autoimmune Disorders
0.72
DOIOpen Access PDFOpenAlex
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Technical challenges of intracellular flow cytometry-based assays as a functional complement to diagnosis of signaling defects of inborn errors of immunity: PI3K pathway as a case of study — Keren Reche Yebra, Arnaldo Bernal, et al. · Frontiers in Immunology (2024) | TGRS Research Map | TGRS