MECHANISM OF CELASTRUS ORBICULATUS EXTRACT INHIBITING THE INVASION AND METASTASIS OF GASTRIC CANCER CELLS THROUGH RAC1/LIMK1/COFILIN 1 PATHWAY

Celastrus orbiculatus extract contains various biologically active components which have extensive medicinal value.The present work investigated the inhibitory mechanism of Celastrus orbiculatus extract on the invasion and metastasis of gastric cancer (GC) cells (GCCs), with particular emphasis on the function of the ras-related C3 botulinum toxin substrate 1 (Rac1)/LIM domain kinase 1 (LIMK1)/cofilin1 signalling pathway (SPW) in this process.First, celastrin was extracted from Celastrus orbiculatus using an ultrasonic extraction (ULE) method and detected by high-performance liquid chromatography (HPLC).The human GCC line MGC-803 was then used in the study.The prepared Celastrus orbiculatus samples showed the same chromatographic peak as the celastrol standard.In contrast to the Ctrl group, the cell proliferation rates (CPRs) and cell invasion rates (CIRs) in groups A, B and C were greatly reduced, while the cell apoptosis rates (CARs) and cell migration rates (CMRs) were greatly increased (p < 0.05).In addition, the Rac1/LIMK1/cofilin1 SPW-associated proteins in the Celastrus orbiculatus treatment groups were strongly downshifted compared to the Ctrl group (p < 0.05).Meanwhile, there was a continuous downward trend with increased DC and time.Celastrus orbiculatus extract, by regulating the Rac1/LIMK1/cofilin1 SPW, suppressed proliferation, invasion and migration of GCCs while facilitating their apoptosis.This discovery provided important evidence for the potential use of Celastrus orbiculatus extract in treating GC. RezumatExtractul de Celastrus orbiculatus conine multiple componente biologic active cu efecte terapeutice.Studiul a investigat mecanismul inhibitor al extractului de Celastrus orbiculatus asupra invaziei i metastazelor celulelor canceroase gastrice (GCC), cu accent deosebit pe funcia substratului 1 (Rac1)/LIM al toxinei botulinice C3 legat de ras 1 (LIMK1)/calea de semnalizare cofilin1 (SPW) n acest proces.n primul rnd, celastrin a fost extras din Celastrus Orbiculatus folosind o metod de extracie cu ultrasunete (ULE) i identificat prin cromatografie lichid de nalt performan (HPLC).Linia GCC uman MGC-803 a fost utilizat n studiu.Probele au prezentat acelai vrf cromatografic ca standardul de celastrol.Spre deosebire de grupul Ctrl, ratele de proliferare celular (CPR) i ratele de invazie celular (CIR) din grupurile A, B i C au fost mult reduse, n timp ce ratele de apoptoz celular (CAR) i ratele de migrare celular (CMR) au fost mult crescute (p < 0,05).n plus, proteinele asociate cu Rac1/LIMK1/cofilin1 SPW din grupurile de tratament cu Celastrus orbiculatus au fost puternic reduse n comparaie cu grupul Ctrl (p < 0,05).Extractul de Celastrus orbiculatus, prin reglarea SPW Rac1/LIMK1/cofilin1, a suprimat proliferarea, invazia i migrarea GCC facilitnd, n acelai timp, apoptoza acestora.