A QUALITY-BY-DESIGN APPROACH FOR OPTIMISATION OF DOCETAXEL TRANSFEROSOMAL FORMULATIONS

This research investigated the design, characterization and optimization of docetaxel-loaded transfersomes for enhanced transdermal delivery in the treatment of skin cancer.A systematic formulation development approach, guided by the principles of Design of Experiments (DoE) and One Factor At a Time (OFAT), was employed to optimize the formulation parameters.Physicochemical properties including particle size, polydispersity index (PDI), zeta potential and entrapment efficiency were comprehensively assessed to ensure the stability and drug-loading capacity of the transfersomes.Morphological analysis using Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) confirmed well-defined transfersome structures.In vitro release kinetics studies demonstrated a controlled and sustained release profile of Docetaxel from the transfersomes, highlighting its potential for enhanced therapeutic efficacy. RezumatAceast cercetare are la baz proiectarea, caracterizarea i optimizarea transferozomilor cu docetaxel pentru o eliberare transdermic mbuntit n tratamentul cancerului de piele.O abordare sistematic de dezvoltare a formulrii, ghidat de principiile Designului de Experimente (DoE) i a modificrii unui singur parametru o dat (OFAT), a fost utilizat pentru optimizarea parametrilor formulrii.Proprietile fizico-chimice, inclusiv dimensiunea particulelor, indicele de polidispersie (PDI), potenialul zeta i eficiena de ncapsulare, au fost evaluate exhaustiv pentru a asigura stabilitatea i capacitatea de ncrcare a medicamentului a transferozomilor.Analiza morfologic folosind microscopia electronic de scanare (SEM) i microscopia electronic de transmisie (TEM) a confirmat structuri bine definite ale transferozomilor.Studiile de cinetic a eliberrii in vitro au demonstrat un profil de eliberare controlat i susinut al docetaxelului din transferozomi, evideniind potenialul su pentru o mbuntirea eficacitii terapeutice.