Understanding the Relationship between MCP-1 SNP (rs1024611) and VEGF SNP (rs699947) with Aging and Lifestyle Habits via Computational Insights

Background: Aging is a complex biological process and is characterized by a gradual decline in physiological functions and increased vulnerability to various diseases. It is linked with the genes known as Monocyte Chemoattractant Protein-1 (MCP-1) and Vascular Endothelial Growth Factor (VEGF). MCP-1 is a key protein in generating immune response and VEGF is involved in angiogenesis. Objectives: This is a cross-sectional experimental study that aims to observe interplay between MCP-1 SNP (rs1024611) and VEGF SNP (rs699947) with aging and lifestyle habits. It delves into the biological pathways implicated in aging, as well as the impact of lifestyle elements such as dietary habits, tobacco use and physical activity on MCP-1 and VEGF expression levels. Method: The study involved the selection of 60 samples from elderly individuals and 60 samples from healthy individuals as control. The selection process was done by filling the proforma having questions related to the lifestyle habits and the use of medication since they can interfere with gene functioning. After that DNA extraction, and the identification of gene presence through PCR analysis. The assessment of PCR outcomes revealed a prevalence of the CC allele in both the experimental and control cohorts for both MCP-1 and VEGF, highlighting the critical role of these genes in the aging process. Furthermore, it posits that variations in the VEGF gene may hold significant sway over aging. Bioinformatics techniques were used to explore the relationship between MCP-1 and VEGF, employing Cytoscape STRING, KEGG pathway analysis and GO enrichment to analyze the interactions between these two genes. Results: It was observed that these genes are interconnected, functioning under the influence of the Tp65 transcriptional factor. The research findings also put forth the notion that dietary habits and physical exercise could potentially modulate the expression of these genes, thereby influencing the process of healthy aging. Those who adopted healthy lifestyle showed CC and TT genotypes for MCP-1 and VEGF respectively therefore, showing slow and healthy aging. Conclusions: The conclusion of this article suggests that the MCP-1 and VEGF genes play a crucial role in immune response, inflammation and aging. The study highlights the potential interaction between these genes in monocyte recruitment and angiogenesis.