Metagenomics Data of a Historical Citrus Psorosis Virus Isolate Infecting Citrus in Australia and its Findings for RT-qPCR Detection

Citrus psorosis virus (CPsV) is a graft-transmissible citrus virus and is the presumed causal agent of citrus psorosis disease. Australian historical isolate CL222 induces symptoms typically associated with psorosis of oakleaf patterns and leaf flecking on inoculated Symons sweet orange biological indicator plants. Genomic analysis of CL222 revealed a nucleotide match of 99.8 to 99.9% to Chinese CPsV genomes (CH3 isolate; GenBank accessions MG673944, MG673945, and MG673946). The detection of a single nucleotide variation in the coat protein gene of the Australian isolate CL222 led to the design of an improved RT-qPCR probe to enable virus detection of Australian and other isolates. CPsV is rarely detected in field trees in Australia, likely due to the widespread use of pathogen-free propagation material. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .