Protocol for generation of PD modeling induced neurons and detection of α-synuclein forms
Alpha-synuclein (α-Syn) is an important molecule in the pathogenesis of Parkinson's disease and Alzheimer's disease-related dementias such as Lewy body dementia, forming multiple pathological species. In vitro disease models, including human neurons and α-Syn-transfected cells, are instrumental to understand synucleinopathies or test new therapies. Here, we provide a detailed protocol to generate human neurons derived from induced pluripotent stem cells (iPSCs), and HEK cells, with α-Syn mutations. We also describe multiple assays to determine the various α-Syn forms.
Authors
- Bo Liang (ORCID: https://orcid.org/0000-0001-8186-5580)
- Francesco Nardi (ORCID: https://orcid.org/0000-0003-0271-9855)
- Gulimiheranmu Maisumu
- You Zhou (ORCID: https://orcid.org/0000-0002-9783-1868)
- Abraam M. Yakoub
Institutions
- Brigham and Women's Hospital (US)
- University of North Dakota (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-11-15
- DOI
- https://doi.org/10.1016/j.xpro.2024.103447
- Citations
- 1
- Primary Topic
- Parkinson's Disease Mechanisms and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.37
Funders
- Foundation for the National Institutes of Health
- Massachusetts Institute of Technology
- Northwestern University
- National Institutes of Health
- Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology