Bio-delignification and Pre-treatment of Oil Palm Frond (OPF) by Trametes Polyzona for Enhanced Biochemical Methane Potential (BMP)

This study aims to optimize pre-treatment conditions for oil palm fronds (OPF) using white rot fungi (WRF) species, Trametes Polyzona for enhancement of biogas production via biochemical methane potential (BMP) test. BMP tests were run under three conditions: OPF alone (control), OPF treated with Trametes Polyzona, and OPF treated with Trametes Polyzona and supplement of water. Visual structures, FTIR, Klason method and GCMS were used to investigate the effect of pre-treatments conditions on bio-delignification process of OPF. Visual structures results showed preferential degradation on OPF by Trametes Polyzona under both dry and moisture conditions compared to that of control conditions. After 30-days of incubation period, 22.5% lignin content in OPF coupled with Trametes polyzona pre-treatment (25-35% reduction), underscores biogas generation potential. Notably, the combined Trametes polyzona and water pre-treatment achieved a remarkable 35% lignin reduction. Both these conditions showed successful lignin degradation, highlighted by FTIR's carbonyl group reduction.