A single NLR gene confers resistance to leaf and stripe rust in wheat
Nucleotide-binding leucine-rich repeat (NLR) disease resistance genes typically confer resistance against races of a single pathogen. Here, we report that Yr87/Lr85, an NLR gene from Aegilops sharonensis and Aegilops longissima, confers resistance against both P. striiformis tritici (Pst) and Puccinia triticina (Pt) that cause stripe and leaf rust, respectively. Yr87/Lr85 confers resistance against Pst and Pt in wheat introgression as well as transgenic lines. Comparative analysis of Yr87/Lr85 and the cloned Triticeae NLR disease resistance genes shows that Yr87/Lr85 contains two distinct LRR domains and that the gene is only found in Ae. sharonensis and Ae. longissima. Allele mining and phylogenetic analysis indicate multiple events of Yr87/Lr85 gene flow between the two species and presence/absence variation explaining the majority of resistance to wheat leaf rust in both species. The confinement of Yr87/Lr85 to Ae. sharonensis and Ae. longissima and the resistance in wheat against Pst and Pt highlight the potential of these species as valuable sources of disease resistance genes for wheat improvement.
Authors
- Hanan Sela (ORCID: https://orcid.org/0000-0002-4218-4376)
- Brande B. H. Wulff (ORCID: https://orcid.org/0000-0003-4044-4346)
- Gerit Bethke (ORCID: https://orcid.org/0009-0009-8431-6419)
- Y. Anikster
- Amir Sharon (ORCID: https://orcid.org/0000-0002-6045-9169)
- Martin Mascher (ORCID: https://orcid.org/0000-0001-6373-6013)
- E. Millet
- Or Sharon (ORCID: https://orcid.org/0000-0003-1299-6127)
- Udi Landau
- Gary J. Muehlbauer (ORCID: https://orcid.org/0000-0001-9320-2629)
- Smadar Ezrati
- Tamar Eilam
- Mahmoud Said (ORCID: https://orcid.org/0000-0002-6169-8655)
- Juan J. Gutiérrez-González (ORCID: https://orcid.org/0000-0002-6795-6192)
- J. Manisterski
- Oadi Matny (ORCID: https://orcid.org/0000-0002-8447-2886)
- Moshe Ronen (ORCID: https://orcid.org/0000-0002-4817-6078)
- Davinder Sharma (ORCID: https://orcid.org/0000-0002-4438-4507)
- Jaroslav Doležel (ORCID: https://orcid.org/0000-0002-6263-0492)
- Anna Minz‐Dub (ORCID: https://orcid.org/0000-0001-5821-1116)
- Manas Ranjan Prusty (ORCID: https://orcid.org/0000-0003-2704-505X)
- Yong Liang (ORCID: https://orcid.org/0000-0002-3702-3113)
- Arava Shatil‐Cohen
- Kateřina Holušová (ORCID: https://orcid.org/0000-0002-4531-7170)
- Pnina Ben-Yehuda
- Raz Avni (ORCID: https://orcid.org/0000-0002-0801-5093)
- Brian J. Steffenson (ORCID: https://orcid.org/0000-0001-7961-5363)
- Matthew Moscou (ORCID: https://orcid.org/0000-0003-2098-6818)
- Helen J. Brabham (ORCID: https://orcid.org/0000-0001-7132-5011)
- Oxana Maatuk
- Rakesh Kumar (ORCID: https://orcid.org/0000-0003-0811-5657)
- István Molnár (ORCID: https://orcid.org/0000-0002-7167-9319)
- Guotai Yu
- Sofia Khazan-Kost
Institutions
- John Innes Centre (GB)
- University of Minnesota (US)
- Tel Aviv University (IL)
- Norwich Research Park (GB)
- Sainsbury Laboratory (GB)
- German Centre for Integrative Biodiversity Research (DE)
- 2Blades Foundation (US)
- Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) (DE)
- Western Regional Research Center (US)
- HUN-REN Centre for Agricultural Research (HU)
- Czech Academy of Sciences, Institute of Experimental Botany (CZ)
- Cereal Disease Laboratory (US)
- Agricultural Research Center (EG)
- Agricultural Institute (HU)
- King Abdullah University of Science and Technology (SA)
- Universidad de León (ES)
- University of Haifa (IL)
Publication Details
- Journal
- Nature Communications
- Published
- 2024-11-15
- DOI
- https://doi.org/10.1038/s41467-024-54068-6
- Citations
- 54
- Primary Topic
- Plant pathogens and resistance mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 32.38
Funders
- U.S. Department of Agriculture
- UK Research and Innovation
- Tel Aviv University
- Council for Higher Education
- Directorate for Biological Sciences
- Agricultural Research Service
- Biotechnology and Biological Sciences Research Council
- European Regional Development Fund