Dysfunction and Morphological Involvement of Inner Macular Layers in Glaucoma

<b>Objectives:</b> This study aimed to study the inner retina functional and morphological impairment of retinal ganglion cells (RGCs) from specific macular rings and sectors to identify whether selective macular regions were more vulnerable than others within the 20 central degrees in patients with open-angle glaucoma (OAG). <b>Methods:</b> In total, 21 OAG patients [mean age 50.19 ± 7.86 years, Humphrey Field Analyzer (HFA) 24-2 mean deviation (MD) between -5.02 and -22.38 dB, HFA 10-2 MD between -3.07 and -17.38 dB], providing 21 eyes, were enrolled in this retrospective case-control study. And 20 age-similar healthy subjects, providing 20 eyes, served as controls. The multifocal photopic negative response (mfPhNR) response amplitude density (RAD) from concentric rings and macular sectors and ganglion cell layer thickness (GCL-T) assessed by Spectral Domain-Optical Coherence Tomography (SD-OCT) was measured. Mean RAD and GCL-T values were compared between OAG and control ones by ANOVA. In OAG eyes, the relationship between mfPhNR and SD-OCT data was examined by linear regression analysis, and Pearson's correlation coefficients were computed. <b>Results:</b> In considering all rings and sectors, compared to the controls, the OAG group showed a significant (<i>p</i> < 0.01) reduction in mean mfPhNR RAD and in GCL-T values with the greatest reduction in the central area. In OAG eyes, a significant (<i>p</i> < 0.01) correlation between all mfPhNR RAD and GCL-T values, with significant (<i>p</i> < 0.01) correlation coefficients, were found. <b>Conclusions</b>: In OAG eyes, RGC dysfunction was detectable by abnormal mfPhNR responses in localized macular areas, mainly in the central one. Localized macular RGC dysfunction was linearly correlated with the GCL morphological changes.