2′-Fucosyllactose improved muscle health in middle-aged mice performing forced swimming exercise

Exercise is effective in improving muscle health, but it is crucial to consume appropriate dietary supplements along with exercise to boost its effects. 2′-Fucosyllactose (2′-FL) combined with forced swimming exercise notable reinforced muscle mass, thickness, and strength of calf muscle compared to the exercise alone. 2′-FL ameliorated exercise-induced oxidative stress, restored antioxidant enzyme activity, and mitigated muscular cell damage. • 2′-Fucosyllactose (2′-FL) combined with forced swimming enhances muscle mass, thickness, and strength of calf muscle compared to exercise alone. • 2′-FL has gene regulatory effects on protein synthesis and muscle growth. • 2′-FL ameliorated exercise-induced oxidative stress, restored antioxidant enzyme activity, and mitigated muscular cell damage. Muscle mass and strength gradually decline from middle age, and appropriate physical activity and exercise habits during this period play a key role in preventing sarcopenia. Exercise is the most effective method for improving muscle health, and consuming appropriate dietary supplements along with exercise can boost its effects. Human milk is the most essential source for muscle growth during infancy, and 2′-fucosyllactose (2′-FL) is most abundant oligosaccharide in human milk. Therefore, we hypothesized that 2′-FL would exhibit muscle protective properties in 10-month-old aged male mice subjected to forced swimming exercises. Our findings revealed that 2′-FL administration significantly improved muscle mass, thickness, and strength of calf muscle compared to forced swimming exercise alone. Additionally, 2′-FL enhanced the hypertrophic changes in gastrocnemius and soleus. Furthermore, 2′-FL upregulated gene expression related to muscle protein synthesis ( PI3K, AKT1 ) and muscle growth ( A1R, TRPV4 ), while inhibiting gene expression related to muscle protein degradation ( Atrogin-1, MuRF-1 ) and muscle growth inhibition ( Myostatin, SIRT1 ). 2′-FL also ameliorated exercise-induced oxidative stress, restored antioxidant enzyme activity, and mitigated muscular cell damage. These findings suggest that 2′-FL may serve as a functional dietary supplement combined with exercise for muscle health.