Physiological effect of leptin and time-restricted feeding-in reproductive value of female rat

The female must expend far more energy during reproduction than the male needs. Given that reproduction requires energy utilization, it makes sense that physiological control mechanisms associated with appetite and nutrition would also be connected. This research evaluated leptin's capacity to alleviate the effects of time-restricted feeding (TRF) in female. Twenty-four adult female rats, weighted 175-200g were divided randomly into four groups; the control group (G1) was given DW, and the (G2) group injected i.p. leptin hormone (50µg/kg BW) with normal diet. (G3) TRF group regimen from (8:00 a.m.-3:00p.m.) diet with water free, (G4) TRF with leptin groups injected i.p. leptin (50 µg/kg BW) regimen from (8:00 a.m.-3:00p.m.) diet with water-free. At the end of the research (8 weeks), Rats were sacrificed serum collection for FSH and LH estimation using ELIZA technology, Ovaries were processed for hematoxylin and eosin staining. Results showed that decreased significantly P≤0.05 in body weight in time- restricted feeding (TRF) with leptin, leptin and TRF, decreased significantly P≤0.05 in ovarian weight of leptin and TRF with leptin. FSH and LH hormone increased significantly P≤0.05 in the leptin group, TRF and TRF with leptin. Histological changes in the treated group showed many primary and secondary follicles with many large antral follicles and corpora luteum, and TRF showed normal follicles. In conclusion, time- restricted feeding promotes the reproductive function of female rats with ovarian hormones and leptin stimulates FSH and LH hormones. This is probably the agent that brings about the beginning of puberty.