Microscopical and molecular diagnosis of canine babesiosis in stray dogs in Erbil, Iraq

Canine babesiosis, or tick-borne protozoal disease, is prevalent among canines globally and is associated with anemia and potentially fatal illnesses in both dogs and wild animals. This research investigated babesiosis in stray dogs from Erbil Province, Iraq, employing microscopic and molecular detection practices. One hundred forty-two blood samples had been collected from stray dogs brought to private clinics and the Erbil dog shelter. Along with the samples, information regarding age group and gender was recorded. Initially, the blood samples underwent examination using the Giemsa-stained blood smear technique. Subsequently, DNA extraction was performed, and the identification of Babesia spp. was confirmed by amplifying the 18S and 28S rRNA genes. The overall incidence was 6.3% using microscopic techniques and 15.5% using PCR. Common clinical symptoms in infected dogs were fever, pale mucous membranes, anorexia, and depression. Molecular testing revealed a significantly greater prevalence of babesiosis in younger dogs 25.5% and female dogs 20.6% compared to older and male dogs. A significant difference was also found between tick infestation and poor body condition. A Phylogenetic study revealed that the two Babesia spp. from the current research with accession numbers OR896912 and OR896913 B. vogeli and OR896867 and OR896868 B. gibsoni have indicated 98-99% similarity to species isolated in India, China, and Brazil. To summarize, the findings underscore the prevalence of canine babesiosis in the region, potentially offering insights to aid in its control measures.