Accelerating the healing of full-thickness excision wounds in mice using piezoelectric direct discharge plasma

Piezoelectric Direct Discharge (PDD) plasma is a cold plasma with a floating electrode. It is a rapidly expanding and emerging wound treatment and tissue repair technology. The wound healing process is complex and requires advanced techniques to restore damaged skin. The current study utilized PDD plasma to heal full-thickness skin wounds in a mice model. The punch-biopsy created two circular wounds with a diameter of approximately 8 mm on the back, and three distinct groups of 12 healthy male mice, aged approximately 3 months, were utilized. The groups were exposed to a high voltage at 13 kV and a high frequency at 4.2 kHz plasma for 10 and 30 seconds, respectively. In contrast, the third group was categorized as the negative control. The findings demonstrate that PDD plasma exhibits notable efficacy in wound healing. The groups treated with plasma experienced a significantly accelerated healing process compared to the control group. The contraction percentage of healing on day 11 was 32.09% for the control group, 79.29, and 96.81% for the other two treated groups, 10 and 30 sec, respectively. The histological examination of skin tissue revealed enhanced tissue regeneration, reduced scarring due to developing angiogenesis, decreased inflammation, and improved treatment efficacy when the exposure time to plasma is increased. This method can be promising in adopting plasma PDD for being inexpensive and effective in promoting wound healing without side effects. This technology combines durability and ease of use.