Serological and molecular detection of <i>Toxoplasma gondii</i> in cats in Europe with evaluation of associated risk factors for pathogen contact/infection

Breed, age, sex, neuter status, seasonality and regionality significantly impacted serological results. Neuter status, seasonality and regionality significantly impacted the PCR results. Immunosuppression (FeLV/FIV) had a significant impact on serological results. PCR-positive cats shed oocysts and spread infection to other susceptible hosts, including humans. Surveillance is therefore recommended, taking into consideration the associated risk factors.