Impact of Some Natural Resources on Biological Aspect of Tuta absoluta M. Under Laboratory and Green House Conditions With Reference to its Enzymatic Activity

This study was targeted to test four vegetable oils namely (Ruta chalepensis, Azadirachta indica, Simmondsia chinensis, Nigella sativa K.L.) against tomato leaf miner Tuta absoluta under laboratory and field conditions, in addition to record its effect on their enzymatic activities. The data illustrated that N. sativa and R. chalapensis caused the highest reduction in eggs hatchability, mortality larval instars and moderate effect in larval penetration under laboratory condition. On other side, the field evaluation was noticed that the most effective one was S. chinensis followed by A. indica and R. chalepensis which recorded reduction in insect infestation reach to ≥ 72 % on sprayed green part. On other hand, the least fruit infestation was recorded in case of treatments by R. chalepensis followed by S. chinensis, A. indica and N. sativa. The enzymatic analysis was given spot light on the reason of the variation in the effect among the tested oils toward 4th larval instars. Data was showed that the compounds reduced the enzymatic activities, which might suggest a poor defense mechanism in the detoxification of the used oils.