Analysis of metabolites associated with ADIPOQ genotypes in individuals with type 2 diabetes mellitus
Diabetes mellitus (DM) is a significant public health problem and it is known that the identification of molecular markers involved in glycemic control can impact disease control. Although the rs266729 polymorphism located in the promoter of the adiponectin gene (ADP) has been shown to be a candidate for involvement in glycemic control, the genotypic groups have never been characterized in terms of metabolomic aspects. Objective: Analyze the metabolites present in the rs266729 genotype groups. 127 diabetic individuals were compared according to the rs266729 genotype groups CC and GC + GG (RFLP-PCR). Blood plasma metabolites were classified by nuclear magnetic resonance (NMR), and the metabolic pathways of each group using the MetaboAnalyst tool. Insulin therapy (p = 0.049) was more frequent in the GC + GG rs266729 group. Lactate, alanine, glutamine, aspartate, lipid, lysine, isoleucine, citrulline, cholesterol, and fucose impacted the CC group and aspartate, beta-glucose, glutamate, pyruvate, proline, and 2-oxoglutarate impacted the CG + GG group. The glucose-alanine pathway, malate-aspartate transport, and urea cycle impacted the CC group (D-glucose, glutamic acid, L-alanine, oxoglutaric acid, and pyruvic acid). The glutamine/glutamate ratio is likely to be related to the causes of rs266729 influencing the risk of diabetes.
Authors
- Bruno Rafael Virgínio de Sousa (ORCID: https://orcid.org/0000-0003-2026-5025)
- Kamila Sabino Batista (ORCID: https://orcid.org/0000-0001-5766-8595)
- Darlene Camati Persuhn (ORCID: https://orcid.org/0000-0001-5291-5454)
- Anauara Lima e Silva (ORCID: https://orcid.org/0009-0003-1765-3008)
- Carla Patrícia Novaes dos Santos Fechine (ORCID: https://orcid.org/0000-0002-2736-9006)
- Yuri Mangueira do Nascimento (ORCID: https://orcid.org/0000-0002-9732-6191)
- Tainá Gomes Diniz (ORCID: https://orcid.org/0000-0002-2211-1858)
- Caroline Severo de Assis (ORCID: https://orcid.org/0000-0003-0525-1418)
- Josean Fechine Tavares (ORCID: https://orcid.org/0000-0003-0293-2605)
- Evandro Ferreira da Silva (ORCID: https://orcid.org/0000-0001-5948-6402)
- Alexandre Sérgio Silva (ORCID: https://orcid.org/0000-0003-3576-9023)
- Mussara Gomes Cavalcanti Alves Monteiro
- Isabella Wanderley de Queiroga Evangelista (ORCID: https://orcid.org/0000-0003-1127-718X)
- Marina Gonçalves Monteiro Viturino
Institutions
- Universidade Federal da Paraíba (BR)
- Hospital Universitário Lauro Wanderley (BR)
- Centro Universitário de João Pessoa (BR)
Publication Details
- Journal
- Scientific Reports
- Published
- 2024-11-15
- DOI
- https://doi.org/10.1038/s41598-024-79686-4
- Primary Topic
- Adipose Tissue and Metabolism
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Fundação de Apoio à Pesquisa do Estado da Paraíba
- Universidade Federal da Paraíba
- Pró-Reitoria de Pesquisa, Universidade Federal do Rio Grande do Sul