Application of novel AIE materials in fluorescence probes

Fluorescence bioimaging is considered an indispensable technique in biological research due to its high sensitivity, excellent spatial and temporal resolution, non-invasiveness, rapid and real-time responsiveness, as well as its ease of accessibility. Among these, fluorescence probes play a pivotal role in fluorescence imaging technology thanks to their strong specificity, high sensitivity, rapid response, and straightforward implementation. Aggregation-Induced Emission (AIE) molecules exhibit a unique phenomenon: they emit weak or no fluorescence when dissolved in a solution but become highly luminescent under aggregated/clustering conditions. Leveraging this, fluorescent probes with AIE characteristics often demonstrate fluorescence activation under conditions of spontaneous aggregation or binding with analytes, showcasing high sensitivity and exceptional signal-to-noise ratios. Consequently, fluorescence probes based on the distinctive optical properties of AIE hold immense potential for applications in fluorescence bioimaging. This paper primarily investigates the synthesis properties and applications in biosensing of a series of novel organic fluorescence probes with aggregation-induced emission (AIE) characteristics. By synthesizing a range of compounds with AIE properties and experimentally determining their photophysical properties and molecular structures, the study explores the potential applications of these compounds in DNA recognition, protein detection, small molecule recognition, cellular pH detection, and bioimaging.