Differential effects of prolonged post-fixation on immunohistochemical and histochemical staining for postmortem human brains
Taken together, these findings suggest that prolonged post-fixation has both positive and negative effects, while age and PMI exert limited influence on these IHC and HC parameters. Therefore, it is essential to consider these differential changes when interpreting results derived from tissues with extended post-fixation durations. Furthermore, if feasible, we recommend conducting IHC and HC staining on human brains with the same post-fixation time spans and using the most optimal antibodies to mitigate the impact on subsequent analyses.
Authors
- Josefina Maranzano (ORCID: https://orcid.org/0000-0002-9696-5522)
- Yashar Zeighami (ORCID: https://orcid.org/0000-0002-0583-5811)
- Éve‐Marie Frigon
- Mahsa Dadar (ORCID: https://orcid.org/0000-0003-4008-2672)
- Weiya Ma
Institutions
- Montreal Neurological Institute and Hospital (CA)
- Douglas Mental Health University Institute (CA)
- McGill University (CA)
- Université du Québec à Trois-Rivières (CA)
Publication Details
- Journal
- Frontiers in Neuroanatomy
- Published
- 2024-11-13
- DOI
- https://doi.org/10.3389/fnana.2024.1477973
- Citations
- 3
- Primary Topic
- Neuroinflammation and Neurodegeneration Mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.74
Funders
- Canadian Institutes of Health Research
- Natural Sciences and Engineering Research Council of Canada
- Fonds de Recherche du Québec - Santé