GC-MS Analysis and ADMET Properties of Tom Brown Weaning Meal and its Subsequent Effect on Liver Enzymes and Learning/Memory Parameters in Male Wistar Rats: A Docking and in Vivo Study

Background: The paucity of information on the effect of Tom Brown's weaning meal on liver and learning and memory functions necessitated this study. Methods: Fifteen rats were acclimatized for a week and used for this study. They were divided into control, combined, and Tom Brown experimental groups. Rat Chow, Rat Chow/Tombrown, and Tom Brown Feed alone were given to the experimental animals accordingly. At the end of the four-week feeding period, liver enzymes (AST, ALT, and ALP) and learning and memory parameters were assessed. GC-MS and ADMET properties were done for Tom Brown and its ligands. Eleven Ligands with zero violations using the Lipinski rule of five (ROF) were docked with netrin, AST, and ALT. Results: ALT, AST, and ALP results of the control, combined, and Tom Brown experimental groups presented as mean ± SEM were 67.89 ± 3.15 Iu/L, 71.68 ± 1.30 Iu/l, and 73.65 ± 0.89 Iu/l; 129.81 ± 1.77 Iu/L, 129.51 ± 1.84 Iu/L, and 130.94 ± 1.31 Iu/L; 22.10 ± 1.24 Iu/L, 23.28 ± 0.61 Iu/L, and 22.48 ± 1.29 Iu/L, respectively. There was no significant difference among experimental groups in liver enzymes or other parameters assessed in this study (P > 0.05). 5-hydroxymethyl furfural and carpaine were ligands with a better docking score. Conclusions: The non-significant values of liver and long-term memory parameters is evident of the meal having no effect on these parameters. 5-hydroxymethyl furfural and carpaine are possible compounds that could enhance liver and leraning/memory functions from docking results. However, they had low peak areas from GCMS result and this effect was not seen.