Evaluation of the potential anti-arthritic effects and evaluation of acute oral toxicity of the active fraction of <i>Eclipta prostrata</i> leaves in rat model

The primary goal of this research was to isolate and assess the bioactive compounds within <i>Eclipta prostrata</i> Linn's chloroform extract, with a focus on their potential to combat arthritis, validating its traditional use. Initially, antioxidant characteristics were evaluated, followed by fractionation through column chromatography. The <i>in-vivo</i> safety assessment, following OECD TG 425 Guidelines, showed no mortality at doses up to 2000 mg/kg, confirming the fraction's safety. Subsequently, the active fraction was assessed in a complete Freund's adjuvant-induced arthritis model. It demonstrated a dose-dependent inhibitory effect on inflammation and improved various parameters, including thermal latency time, dorsal flexion, motility, and motor coordination. Active bio constituents like rutin, stigmasterol, quercetin, and beta-sitosterol were estimated in the active fraction. The findings provide validation for the traditional use of <i>E. prostrata</i> as a potential plant with anti-arthritic properties, suggesting its potential suitability for rheumatoid arthritis treatment.