Influence of some Alu insertion sequences (insertion/deletion) variations in families discrimination

The current study focused on the use of Alu elements in determining identity among individuals and they were effective for determining identity, Sixty blood samples were collected, from 14 families in Babylon Governorate to study the identification of people based on the Alu elements, where 4 sites were identified, (2q21.1), (8q23.1), (13q34) and (15q23). A PCR device was used to amplify the required sites. Polyacrylamide migration technique used to separate bands and the results are represented by additions and deletions elements. In site A(2q21.1), the P-value was between (0.04-0.22), the PIC value ranged 0-0.444 and Chi-square (3-8). In site B(8q23.1), the P-value ranged (0.018-0.48) the PIC value (0-0.5) and the Chi-square (1.44-6). In the C(13q34) site, the P-value was (0.049-0.941) PIC value was (0.486-0.5) and Chi-square (1.44-6). In the site D(15q23), the P-value was (0.049-0.941). The PIC value was (0-0.5) and Chi-square (0.04-4) at the site. The observed, expected and allele frequencies percentages were calculated for site A(2q21.1). The addition was (0.75) and deletion was (0.25). Additions and deletions were: site B(8q23.1) (0.375), (0.625). C (13q34) (0.5), (0.5). D (15q23) (0.775), (0.225) respectively and the PIC value ranged 0.5 - 0.37, P-value 0- 0.953 and Chi-square 60-0.08 for all sites and for all samples. Finally, the similarity between individuals (60) ranged between 0-1.