Molecular detection of Pseudomonas aeruginosa isolated from children with urinary tract infections

The purpose of this research was to identify Pseudomonas aeruginosa from pediatric UTI samples using molecular methods. Bacteria were isolated and identified using a primary medium. Children from all around Baghdad city who were hospitalized at a private clinic between 2019 to 2022 and showed symptoms of a urinary tract infection (UTI) such as fever and vomiting, had their urine sampled. There were 132 samples of children's urine collected from kids who had UTIs. The VITEK-2 system was completed on pure bacterial isolates. DNA of these bacteria was done and two primers were used in this study for the detection of P. aeruginosa. The present study showed that the rate of infection by P. aeruginosa was 7.6%, the total Gram-positive bacteria were 22.7% and the total Gram-negative bacteria were 77.3%. It was shown that females had a greater rate of UTI (55.3% vs. 44.7%) than males. In conclusion, because of its prevalence and severity in community-acquired UTIs in children and its unique risk factors and outcomes, P. aeruginosa should be suspected in any child who has recently received or is now receiving antibiotic treatment.