The assessment of the minimum inhibitory concentration of Botulinum toxin on Staphylococcal bacteria isolated from Acne vulgaris and validating its impact through Polymerase Chain Reaction analysis

Acne vulgaris (AV) is an inflammation of pilosebaceous unit which commonly occurs in adolescence. Staphylococcus aureus is also involved in AV pathogenesis. This study included isolating and diagnosing 35 samples of bacteria isolated from acne by the aerobic method and dividing them according to their sensitivity to methicillin,10 samples were used in determining the extent of the effect of different concentrations of Botulinum toxin on bacteria using PCR technology. The results obtained 2.5mg/ml as the MIC for the bactria. Botox treatment at 2.5mg/ml demonstrated molecular effects, causing a lack of PCR binding in selected samples. This suggests a potential disruption in gene transcription or DNA degradation, aligning with international studies showcasing Botox's molecular impact on acne-related Staphylococcus aureus, supporting its role in combination therapy for bacterial control. Botox, renowned for cosmetic use, is also under investigation for medical applications like treating acne. Research suggests that Botox may reduce acne by minimizing sebum production and inhibiting bacteria associated with acne, particularly Staphylococcus aureus. Global studies support these findings, revealing potential applications beyond cosmetics, expanding into acne treatment.