The activity of silver nanoparticles produced by Aerococcus spp. against some Acinetobacter baumannii isolates

Nanoparticles are among the fine materials that have an important role in many medical and health sciences such as medicine, biology, physics, chemistry and others. The purpose of this study was to create silver nanoparticles from Aerococcus spp. then used it against isolates of Acinetobacter baumannii. The method included growing Aerococcus spp in a culture medium. Silver nanoparticles were created when the color of medium changed to brown. Some structural properties of these materials were measured using TEM, FTIR, UV-Vis and XRD to characterize the AgNPs. The results showed the ability of Aerococcus spp to produce silver nanoparticles after adding alkaline silver nitrate solution to the culture medium and then the color change occurred. The biochemical tests showed the ability of bacteria; silver nanoparticles (NPs AgNO3) to absorb ultraviolet radiation at 200 nm. X-ray diffraction test showed that these nanoparticles formed radial peaks of 200, 202 and 311 at a wavelength of 25.94 nm depending on the crystalline state of the particles. The Scanning electron microscope test also showed these nanoparticles forming spherical nanoparticles at size of 32.78 nm. The Ag NPS antibacterial activity shows the ability of these nanoparticles at concentration 250μg/ml against inhibition of the growth of some A. baumannii isolates with an inhibition diameter of 30.32. Finally, the antibiofilm forming test showed the ability of Aerococcus spp. (NPs AgNO3) to reduce biofilm forming of A. baumannii at 100%.