A rhodamine based near-infrared fluorescent probe for selective detection of Cu<sup>2+</sup> ions and its applications in bioimaging
A novel near-infrared fluorescent probe TM2 based on a rhodamine-bearing framework was disclosed with a large Stokes shift (100 nm). TM2 exhibits highly selective recognition for Cu<sup>2+</sup> in EtOH/H<sub>2</sub>O (1 : 1, v/v) solution with a low detection limit (2.3 μM) and a wide detection range (0-50 μM). Detection of Cu<sup>2+</sup> is undisturbed at physiological pH levels of 5-9. This recognition mechanism is attributed to the formation of a 1 : 1 complex between TM2 and Cu<sup>2+</sup>, validated by Job's plot, <sup>1</sup>H NMR titration, and LC-MS experiments. Moreover, the successful fluorescence imaging of Cu<sup>2+</sup> both <i>in vitro</i> and <i>in vivo</i> was also accomplished.
Authors
- Jia‐Hai Ye (ORCID: https://orcid.org/0000-0002-3392-9399)
- Shumeng Li (ORCID: https://orcid.org/0009-0000-0240-1033)
- Yuncong Chen (ORCID: https://orcid.org/0000-0002-8406-4866)
- Zijian Guo (ORCID: https://orcid.org/0000-0003-4986-9308)
- Zhijian Chang (ORCID: https://orcid.org/0000-0003-2415-9051)
- Fuyan Lin
- Xuedong Gong
Institutions
- Nanjing University of Science and Technology (CN)
- Nanjing University (CN)
Publication Details
- Journal
- Analytical Methods
- Published
- 2024-11-13
- DOI
- https://doi.org/10.1039/d4ay01922a
- Citations
- 4
- Primary Topic
- Molecular Sensors and Ion Detection
- Type
- article
- Field-Weighted Citation Impact
- 0.96