4D live tracing reveals distinct movement trajectories of meiotic chromosomes

Proper chromosome alignment at the spindle equator is a prerequisite for accurate chromosome segregation during cell division. However, the chromosome movement trajectories prior to alignment remain elusive. Here, we established a 4D imaging analysis framework to visualize chromosome dynamics and develop a deep-learning model for chromosome movement trajectory classification. Our data reveal that chromosomes follow at least three distinct movement trajectories (retracing, congressing, and quasi-static) to arrive at the equator. We further revealed the distinct roles of multiple kinesin superfamily proteins (KIFs) in coordinating and maintaining the chromosome movement trajectories. In summary, we have presented an efficient and unbiased approach to studying chromosome dynamics during cell division, thereby uncovering a variety of chromosome movement trajectories that precede alignment.

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Publication Details

Journal
Life Medicine
Published
2024-11-13
DOI
https://doi.org/10.1093/lifemedi/lnae038
Primary Topic
Microtubule and mitosis dynamics
Type
article
Field-Weighted Citation Impact
0.00

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article

4D live tracing reveals distinct movement trajectories of meiotic chromosomes

Linjin Chen, Chengqi Lin, Feng Xiong, Jiang Xu et al.
Life Medicine
Microtubule and mitosis dynamics
article

4D live tracing reveals distinct movement trajectories of meiotic chromosomes

Linjin Chen, Chengqi Lin, Feng Xiong, Jiang Xu, Hua Cao, Zhuojuan Luo, Lincui Da, Ke Fang, Peng Xie, Yuanhui Ji, Jin Zhang, Xinrui Wang, Shiqi Zhu, Beihong Zheng, Yan Sun
article en

Abstract

Proper chromosome alignment at the spindle equator is a prerequisite for accurate chromosome segregation during cell division. However, the chromosome movement trajectories prior to alignment remain elusive. Here, we established a 4D imaging analysis framework to visualize chromosome dynamics and develop a deep-learning model for chromosome movement trajectory classification. Our data reveal that chromosomes follow at least three distinct movement trajectories (retracing, congressing, and quasi-static) to arrive at the equator. We further revealed the distinct roles of multiple kinesin superfamily proteins (KIFs) in coordinating and maintaining the chromosome movement trajectories. In summary, we have presented an efficient and unbiased approach to studying chromosome dynamics during cell division, thereby uncovering a variety of chromosome movement trajectories that precede alignment.

Life MedicineVol. 3(6)
Fujian Medical University (CN), Shenzhen University (CN), Nantong University (CN), Southeast University (BD), Fuzhou Maternity and Child Health Care Hospital (CN), Southeast University (CN)
National Natural Science Foundation of China
Openalex Percentile: Top 79%
Microtubule and mitosis dynamics
DOIOpen Access PDFOpenAlex
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