Physiological quality of <i>Passiflora edulis</i> Sims seeds evaluated by different processing methods, storage times and packages

ABSTRACT Maintaining seed vigour during the storage process is essential for the success of germplasm banks of sexually propagated species such as Passiflora edulis . However, the literature is still scarce regarding efficient preservation methods, especially for long periods. The objective of this study was to evaluate the effect of different seed processing methods, types of packaging and storage conditions of Passiflora edulis seeds for a period of five years. Different seed processing methods (natural fermentation and use of a mixer), packaging (Falcon tubes, plastic bags and paper bags), storage temperatures (room temperature, cold chamber and refrigerator) and evaluation times (one, three and five years) were analysed. Seeds stored in a refrigerator and cold chamber maintained high germination (&gt;90%) for up to five years. However, seeds stored at room temperature reduced their viability after one year of storage. No significant variations were observed between the seed processing and packaging methods evaluated. Absence of respiratory activity was confirmed by the tetrazolium test performed on seeds stored for five years at room temperature. To maintain the vigour of P. edulis seeds, it is recommended to store them in a cold room or refrigerator in different packaging and using different methods for seed extraction.