Optical properties of nanoparticles in blood: considering blood absorption

Nanomedicine is emerging as a crucial avenue for exploring new therapeutic and diagnostic techniques in the medical field. Effective monitoring of the dispersion concentration of nanoparticles using optical methods is an extremely important topic in this area. However, existing research has not recognized that the light absorption of blood can lead to significant errors in related optical measurements. This paper, considering the absorption properties of the background medium, meticulously discusses the variations in the single-scattering characteristics of nanoparticles in plasma and whole blood, and analyzes the influence of parameters such as incident wavelength, particle size, refractive index, and background medium refractive index. The results indicate that neglecting the light absorption of the background medium may lead to differences of up to approximately 50% in the results, but this is also influenced by parameters such as incident wavelength, particle size, and refractive index. Furthermore, there are still significant differences in the variations of the single-scattering properties of nanoparticles with identical characteristics in plasma and whole blood. These results indicate the importance of in-depth research into calibration techniques for optical instruments in monitoring nanoparticles in the blood, and further enhance the development of nanoparticle monitoring technology in nanomedicine.