In-vitro and in-silico investigation of anti-obesity effect of Shorea tumbuggaia

The rising prevalence of obesity has become a significant concern in both high income and low income countries. Addressing obesity has challenges due to the limitations in current treatment options. Dietary modifications have become a common approach in managing obesity and its associated risks. The study was aimed atinvestigating the anti-obesity effects of the methanol extract of Shorea tumbuggaia, focusing specifically on its ability to inhibit pancreatic lipase activity. Shorea tumbuggaia stem bark methanolic preparation was screened for total phenolic content and subjected to phytochemical tests for presence of secondary metabolites. The pancreas of a chicken was used to extract the lipase enzyme. Lipase inhibitory activity of various methanol extract concentrations was assessed using olive oil as the substrate. Additional docking investigations of Shorea tumbuggaia components were made to investigate the role in regulating obesity and its associated issues.The study revealed that the extract has exhibited concentration dependent inhibition of pancreatic lipase activity. The study findings on Shorea tumbuggaia lipase inhibitory activity are encouraging which may be due to the existence of secondary metabolites causing the inhibitory action.