Development of triggerable peroxidase-tagged primers for colorimetric DNA detection applicable to various DNA polymerases
Abstract To meet the demand for more cost-effective rapid and sensitive nucleic acid detection methods to benefit various applications, we introduce a novel technique that uses a modified triggerable guanine quadruplex-based peroxidase (POD)-tagged primer. This technique allows sensitive colorimetric detection by activating an inactive POD tag during polymerase chain reaction amplification, which, importantly, functions independently of the type of DNA polymerase used.
Authors
- Masaki Hagihara (ORCID: https://orcid.org/0000-0002-2678-825X)
- Hirohumi Munakata
- Eiji Morita (ORCID: https://orcid.org/0000-0002-3781-6121)
- Kotaro Ishida (ORCID: https://orcid.org/0009-0008-6808-5887)
- Yuri Sohma
- Shin‐ichi Sato (ORCID: https://orcid.org/0000-0003-3085-419X)
- R Ohno (ORCID: https://orcid.org/0009-0005-9972-5013)
- Kozo Utsumi
Institutions
- Hirosaki University (JP)
- Kumamoto University (JP)
Publication Details
- Journal
- Chemistry Letters
- Published
- 2024-11-01
- DOI
- https://doi.org/10.1093/chemle/upae212
- Primary Topic
- Biopolymer Synthesis and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Institute of Advanced Energy, Kyoto University