Characterization of Antimicrobial Activity and Fingerprinting for Disorganized and Organized Cultures in Datura innoxia

Datura innoxia is renowned for its pharmaceutical qualities and traditional medical use. It’s crucial for long-term pharmaceutical drug synthesis by regulated micropropagation and tissue culture techniques. This study focused on establishing Datura innoxia under aseptic laboratory settings to promote callus formation and comparing its phytochemical profile with leaves using High-Performance Liquid Chromatography (HPLC). The goal was to identify potential changes in phytochemical content between various growing strategies. Antibacterial efficacy of extracts from conventional plants and lab-grown callus were evaluated against Escherichia coli and Staphylococcus aureus strains. Moreover, aiming to ascertain the genetic stability of Datura innoxia cells in different conditions, the fingerprinting profile was performed by Start Codon Targeted (SCoT) and Inter-Simple Sequence Repeat (ISSR) markers. By thoroughly examining these features, the study provides insights into the biochemical, pharmacological, and genetic properties of Datura innoxia, which are critical for its use in pharmaceutical research and development. These findings highlight the critical role of regulated cultivation practices in improving the efficacy and sustainability of medicinal plant-based therapies.