Biosynthesis of Selenium and Copper Oxide Nanoparticles using Probiotic Bacteria against Food Spoilage Microorganisms

The study’s aim is to synthesize selenium (SeNPs) and copper oxide (CuONPs) nanoparticles using probiotic Lactobacillus acidophilus as a reducing agent. The biosynthesized probiotic Lactobacillus acidophilus selenium and copper oxide nanoparticles were known by color change. The characterization of SeNPs and CuoNPs was completed by Ultraviolet-visible (UV-VIS) spectroscopy, Field Emission Scanning electron microscope (FESEM), Atomic force microscope (AFM), X-Ray diffraction analysis (XRD) and Fourier transform infrared spectroscopy (FTIR). These tests are utilized for detecting stability, morphology, size, crystalline nature and functional groups on nanoparticles prepared surface. Outcomes revealed appearance of the brick-red and green color, representing a specific color of selenium and copper oxide nanoparticles. It was also disclosed that UV-VIS spectroscopy indicated band absorbance at 236 and 264 nanometer of intense surface Plasmon resonance, manifesting the formation and stability of prepared SeNPs and CuONPs. The FESEM image displayed mulit-shapes between spherical and vaulted for selenium and copper oxide nanosized. XRD at 2 theta revealed crystalline selenium and copper oxide nanoparticles where the average size is 75.52-153.22 nm. FTIR revealed the presence of functional groups of the supernatant that act as stabilizing and reducing agents. The antibacterial activity of synthesized nanoparticles was studied against five different bacteria causing food spoilage. As a result of this study, selenium nanoparticle and copper nanoparticles were biosynthized successfully in less time and easy consuming way by green synthesis of the supernatant probiotic Lactobacillus acidophilus.