Effect of the injection of protoscolex antigens for hydatid cysts on some immunological parameters and the protection of injury

This study aimed to that capability of preparation of antigens from protoscolex found inside the hydatid cyst and likewise possibility of preparing antigen providing protection of the injury of this cysts. Cystic echinococcosis is the more important parasitic disease caused by a cestode tapeworm of species Echinococcus granulosus, spreading a lot in Iraq and the Eastern Mediterranean Countries in addition to its world distribution. The protoscolex found inside hydatid cysts results from the germinal layer of hydatid cyst isolated from people by the surgery section at the Fallujah teaching hospital to find out the extent of its influence on both of the cellular and humeral immunity. 20 white mice (Balb/c) were divided into two groups, the first group of white mice (Balb/ c) was injected subcutaneously with a concentration of 0.1ml of antigen and it measured antibodies IgG, IgA, IgM, IgE as well as injected with complement C3, C4 and the insulation of neutrophil cells and measuring percentage of phagocytosis, reduction of a dye NBT and viability of neutrophil cells and in the second group, the protoscolex was injected in the liver with a concentration 0.1ml to find out the extent of prevention provided by the antigen to prevent or to reduce injury. The results showed a significant increase for all types of antibodies and complements C3 , C4 and reduction of a dye NBT and phagocytosis especially at the time 60 minute and the results were not showing significant differences on viability of neutrophil cells. As for prevention of injury, the results have shown a significant difference at the level < 0.05 and reached the percentage of prevention 56.4 % compared to the subordinate control. The results showed small size of the hydatid cyst in the experience parameters compared to the subordinate control.