Histopathological and immunohistochemical Effects on the Role of Silver Nanoparticles (Ag NPs) on male rats

Background:. The present study was conducted at the Animal House Unit, Biotechnology Research Center, Al-Nahrain University, Iraq, Baghdad.The aim of this study was to focus on the synthesis and characterization of Silver Nanoparticles (Ag NPs) and study the effect of different concentrations of Silver Nanoparticles (Ag NPs) on lipid peroxidation, antioxidant defense system, biochemical parameters, in addition to histology and immunohistochemistry in kidney of male rats. Methods:. Fifty male rats (150-170g) were used. Animals were divided into five groups of 10 rats each. The first group was used as control, while group II, group III, group IV and group V were treated orally with Silver Nanoparticles (Ag NPs) at a dose of 1/150 LD50, 1/100 LD50, 1/50 LD50, and 1/25 LD50 mg/kg BW/day for a period of three weeks. At the end of the experimental period, blood samples, kidney were collected for the investigation of different parameters. Result: . Treatment with Silver Nanoparticles (Ag NPs) different concentrations increased kidney thiobarbituric acid reactive substances (TBARS) and hydrogen peroxide (H2O2) and reduced glutathione (GSH) content as compared to control,Histopathological and immunohistochemical changes were observed and this confirmed the biochemical perturbations occurred due to Silver Nanoparticles (Ag NPs) different concentrations in rat kidney.Conclusively, it is clear that Silver Nanoparticles (Ag NPs) induced pronounced hazardous effects in rat kidney in a dose dependent manner.