Enhancing stability and bioactivity of <i>Chondrus ocellatus</i> polyphenols through nanoparticle fabrication

This article aimed to fabricate Chondrus ocellatus polyphenols (COPs)-gelatin-chitosan nanoparticles to enhance their stability and bioactivity. Different preparation conditions were tested to investigate the effects of formulation on nanoparticle fabrication. Free radical scavenging activity of COPs and their nanoparticles were compared. The consequences revealed that optimal preparation was obtained with a chitosan (CS) concentration of 0.5 mg/mL, gelatin (Gel) concentration of 1.0 mg/mL, COPs concentration of 5.0 mg/mL, and Gel-CS-COPs mass ratio of 2:1:1. The resultant nanoparticles had the particle size of 39.79 ± 5.15 nm and encapsulation efficiency of 60.95 % ± 1.86 %. The COPs-Gel-CS nanoparticles were distributed uniformly, and no obvious aggregation was observed by transmission electron microscopy. Nanoencapsulation of the COPs significantly improved their antioxidative stability. This study provided a potential formulation for the application of Chondrus ocellatus polyphenols in antioxidant activities.