TMOD-34. GENERATION OF BRAIN-VESSEL-GBM ASSEMBLOIDS USING PATIENT-DERIVED GLIOBLASTOMA TISSUE

Abstract Glioblastoma (GBM) is the most common malignant brain tumor of the central nervous system and has a significantly low survival rate of approximately 15 months. Current preclinical GBM models are limited by the lack of a normal human microenvironment and the inability of many tumor cell lines to accurately reproduce GBM biology. To address these limitations, we describe a detailed procedure to generate brain-vessel- GBM assembloids, which are fusions of glioblastoma organoids (GBOs) from resected patient tumor tissue and cerebral and vessel organoids derived from human pluripotent stem cells. The proposed methodology will be used to detect and treat brain tumors, which will enable personalized treatment for brain tumor diseases. By universalizing the methodology we present, we aim to encourage other scientists to further develop existing models for studying these lethal tumors.