Photoresponsive Adenosine Derivatives for the Optical Control of Adenosine A <sub>2A</sub> Receptors in Living Cells
The use of photoresponsive ligands to optically control proteins of interest, known as photopharmacology, is a powerful technique for elucidating cellular function in living cells and animals with a high spatiotemporal resolution. The adenosine A<sub>2A</sub> receptor (A<sub>2A</sub>R) is a G protein-coupled receptor that is expressed in various tissues; its dysregulation is implicated in severe diseases such as insomnia and Parkinson's disease. A detailed elucidation of the physiological function of A<sub>2A</sub>R is, therefore, highly desirable. In the present study, we developed two photoswitchable ligands, photoAd(blue) and photoAd(vio), that target A<sub>2A</sub>R. Using photoAd(vio), we successfully demonstrated the selective activation of A<sub>2A</sub>R in living cells by violet-light irradiation with high spatiotemporal resolution.
Authors
- So Iwata (ORCID: https://orcid.org/0000-0003-1735-2937)
- Tomohiro Doura (ORCID: https://orcid.org/0000-0003-1826-6010)
- Yuma Matsuoka
- Shigeki Kiyonaka (ORCID: https://orcid.org/0000-0002-4100-6738)
- Hidetsugu Asada (ORCID: https://orcid.org/0000-0001-6255-4728)
- Tsuyoshi Araya (ORCID: https://orcid.org/0000-0002-7197-1053)
- Yuya Matsuba
- Harufumi Suzuki
Institutions
- Kyoto University (JP)
- SPring-8 (JP)
- Nagoya University (JP)
Publication Details
- Journal
- ACS Chemical Biology
- Published
- 2024-11-11
- DOI
- https://doi.org/10.1021/acschembio.4c00583
- Citations
- 2
- Primary Topic
- Photochromic and Fluorescence Chemistry
- Type
- article
- Field-Weighted Citation Impact
- 0.23
Funders
- Takeda Science Foundation
- Novartis Foundation
- Nagoya University
- Japan Society for the Promotion of Science
- Exploratory Research for Advanced Technology