On the label-free analysis of white blood cells by holographic quantitative phase imaging flow cytometry
This study presents an innovative methodology to analyze a blood sample from a healthy donor, providing a quantitative characterization of white blood cells (WBCs). It aims to evaluate the effectiveness of holographic quantitative phase imaging (QPI) flow cytometry (FC) in examining phase-contrast maps at the cellular level, thereby enabling the identification and classification of granulocyte types. Additionally, we demonstrate that an unsupervised method can differentiate granulocyte sub-types, i.e., neutrophils and eosinophils. The results instill strong confidence in the potential future use of QPI FC for liquid biopsies and/or for assessing the heterogeneity of WBCs and, more broadly, to facilitate label-free blood tests.
Authors
- Pier Luca Maffettone (ORCID: https://orcid.org/0000-0001-7914-0089)
- Daniele Pirone (ORCID: https://orcid.org/0000-0003-1317-7545)
- Giusy Giugliano
- Achille Iolascon (ORCID: https://orcid.org/0000-0002-9558-0356)
- Francesca Ferranti (ORCID: https://orcid.org/0000-0002-4648-4069)
- Martina Mugnano (ORCID: https://orcid.org/0000-0003-2734-8448)
- Silvia Mari (ORCID: https://orcid.org/0000-0003-1355-8508)
- Annalaura Montella
- Lisa Miccio (ORCID: https://orcid.org/0000-0001-9427-881X)
- Jaromír Běhal (ORCID: https://orcid.org/0000-0001-5537-4091)
- Pietro Ferraro (ORCID: https://orcid.org/0000-0002-0158-3856)
- Pasquale Memmolo (ORCID: https://orcid.org/0000-0002-9607-7728)
- Giulia Scalia (ORCID: https://orcid.org/0000-0002-2721-0134)
- Mario Capasso (ORCID: https://orcid.org/0000-0003-3306-1259)
- Vittorio Bianco (ORCID: https://orcid.org/0000-0003-1956-4976)
- Vincenza Cerbone
- Zhe Wang (ORCID: https://orcid.org/0000-0002-0593-0413)
- Fabrizio Licitra
Institutions
- Agenzia Spaziale Italiana (IT)
- CEINGE Biotecnologie Avanzate Franco Salvatore (Italy) (IT)
- Palacký University Olomouc (CZ)
- University of Naples Federico II (IT)
Publication Details
- Journal
- Journal of the Optical Society of America A
- Published
- 2024-11-11
- DOI
- https://doi.org/10.1364/josaa.536841
- Citations
- 6
- Primary Topic
- Digital Holography and Microscopy
- Type
- article
- Field-Weighted Citation Impact
- 2.03