Stepwise Assembly of DNA Nanostructures in a Surface-Based Method
Hierarchical assembly of DNA nanostructures has already led to superstructures of ever-increasing level of complexity. Processing control in building nanostructures hierarchically is desirable but remains underexplored. Here, we present the stepwise assembly of DNA origami nanostructures by a surface-based method. With solid support of magnetic beads or glass slides, we demonstrate hierarchical assembly of preformed DNA origami units to a number of superstructures. The anchoring of DNA constructs on the surface results in better programmability and controllability for DNA self-assembly, suggesting a potential for our surface-based strategy to become a general and standardized assembly methodology of DNA nanostructures and beyond.
Authors
- Bryan Wei (ORCID: https://orcid.org/0000-0003-2515-2409)
- Huangchen Cui
- Tanxi Bai
- Miao Yu (ORCID: https://orcid.org/0000-0002-3155-5901)
- Yifan Li (ORCID: https://orcid.org/0000-0002-1247-0718)
- Jianbin Wang (ORCID: https://orcid.org/0000-0001-6725-7925)
- Haiyue Wang (ORCID: https://orcid.org/0009-0003-6894-7380)
- Jizhou Li (ORCID: https://orcid.org/0000-0002-1084-2047)
- Yang Yu (ORCID: https://orcid.org/0000-0001-8092-2379)
- Tianqing Zhang (ORCID: https://orcid.org/0000-0002-8783-4416)
- Linfeng Yang
- Yaqi Wang (ORCID: https://orcid.org/0000-0002-6831-9742)
Institutions
- Tsinghua University (CN)
Publication Details
- Journal
- ACS Nano
- Published
- 2024-11-11
- DOI
- https://doi.org/10.1021/acsnano.4c06024
- Citations
- 3
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.70
Funders
- National Natural Science Foundation of China
- Tsinghua University
- National Key Research and Development Program of China